Journal: BMC Immunology

Article Title: The impact of HIV infection on the frequencies, function, spatial localization and heterogeneity of T follicular regulatory cells (TFRs) within human lymph nodes

doi: 10.1186/s12865-022-00508-1

Figure Lengend Snippet: FCRL3 and DPP4 define distinct TFR populations. A Flow cytometry plot showing co-expression of FCRL3 and DPP4 by TFRs. B Representative contour plots demonstrating overlay of TFR populations (grey) and cytokine secreting cells (red) and summary plots showing C IL-2, D TNF-α and E IFN-γ expressing cells within TFR subsets. F Representative flow cytometry plots and G aggregate data depicting co-expression of IL-2 and TNF-α by the TFR subpopulations. H Representative flow plot demonstrating overlay of FCRL3 + TFRs (grey) populations and FOXP3 + (red) cells together with DPP4 + Tfrs (grey) and FOXP3 + cells (red). I Representative image of immunoflouresently stained LN sections with zoomed-in images below, LNs were stained with BCL-6 (green), FOXP3 (red) and DPP4 (white)

Article Snippet: Fixed and permeabilized cells were washed again with perm wash buffer (BD, 519,008,102) and incubated for 20 min at RT with perm wash buffer containing FOXP3- PE-CF594- 259D/CY (BD, 562,421), TNF-α-A700-Mab11 (BD, 557,996), IFN-γ-BV711-45.B3 (BD, 502,540) and IL-2-FITC (BD, 5344.111) antibodies.

Techniques: Flow Cytometry, Expressing, Staining